Annette C. Vergunst

PhD

+33 467028157

Email

ORCID iD : 0000-0002-4359-1491

I am interested in the cellular and molecular mechanisms that underlie pathogenic intracellular bacterial interactions with their host. In my group, we are using the opportunistic Burkholderia cenocepacia as a model bacterium to study both host and bacterial factors that are important for persistent and acute infection using zebrafish embryos.

Johan Gigan

PhD candidate

+33 467028167

Email

My PhD project is a part of an ANR project, called BURKADAPT. I test the virulence of a panel of Burkholderia strains isolated from different ecological niches in the zebrafish model.

My second objective is to use transposon-directed insertion site sequencing (TraDIS, in collaboration with the Sanger Institute) and Tn-Seq to identify Burkholderia cenocepacia genes that are essential for survival and replication in zebrafish macrophages in vivo.

Julien Boyer

Technician – fish facility

+33 467028167

Email

I take care of the zebrafish facility. I am also involved in lab management tasks and research activities in the laboratory.

My favourite meal is fish and chips

Infections of bacteria belonging to the Burkholderia cepacia complex

Bacteria belonging to the Burkholderia cepacia complex (Bcc), including one of the more virulent Burkholderia cenocepacia species, are are a major cause of mortality and morbidity in cystic fibrosis patients. Infection can be asymptomatic but often results in chronic progressive worsening of lung function and sometimes acute fatal necrotizing pneumonia and sepsis, termed Cepacia Syndrome. The inherent resistance of the bacterium to clinically used antibiotics makes it is impossible to control the infection. Opportunistic infections with Bcc bacteria are emerging also in non-CF conditions. The molecular mechanisms that allow B. cenocepacia to cause disease in humans are still largely unknown. B. cenocepacia is an intracellular pathogen. We are using the zebrafish larvae as an infection model to better understand the role of intramacrophage stages on virulence during acute and persistent infections in vivo, and the transition between chronic and acute disease. Our studies focus on host autophagy and pro-inflammatory signaling, and we are using high throughput techniques to identify bacterial and host factors that are critical for the intracellular survival and replication of the bacteria.

Zebrafish embryo infection model for Bcc

We have established the zebrafish (Danio rerio) embryo model to study Bcc virulence. The zebrafish has become a potent biomedical research model that has revolutionized our knowledge of the interaction of microbes with host phagocytes and the immune system through powerful live imaging and genetic tools. The transparent fish embryos allow easy real time analysis of infection with fluorescent bacteria, and in combination with transgenic reporter fish this allows for remarkable imaging of the behavior of host cells during infection. Most importantly, an innate immune system, with a high degree of similarity to that of mammals is already developing in the young embryo.

In zebrafish embryos, the epidemic CF isolate B. cenocepacia K56-2 induces an acute pro-inflammatory infection that becomes rapidly fatal. Intravenously injected bacteria are mainly taken up by macrophages in which they are able to survive and efficiently replicate (Vergunst et al 2010), prior to dissemination and induction of systemic fatal infection. In contrast, other isolates, notably B. stabilis LMG14294 and B. vietnamiensis FC441, cause persistent infection, characterized by low but steady bacterial numbers in macrophages. We have recently found that macrophages are critical for bacterial replication and development of fatal pro-inflammatory responses in zebrafish embryos, in agreement with clinical observations. The model is particularly amenable to study in more detail these intracellular stages in vivo, and the effect of bacterial gene expression on host immune response.

Zebrafish embryo expressing GFP in neutrophils and mCherry in macrophages

We are using the zebrafish embryo as an animal model to study the capacity of intracellular Bcc to arrest maturation of (auto)phagosomes and create an intracellular replication niche, and to study and identify host factors involved in persistent and acute phases of the disease.

  1. Analysis of intracellular bacterial stages and identification of bacterial and host factors involved in infection. 
  2. Analysis of the role of the lysR type transcriptional regulator ShvR in acute infection

Grants

ANR BURKADAPT, Coordinator IRD (Montpellier) (2019-2023)

Marie-Curie International training network (2012-2015)   Appendix_2_FishForPharma_logo

Vaincre la Mucoviscidose (2016-2018), link

Fondation pour la recherche médicale (2010, 2016) link

Région de Languedoc-Roussillon (2010-2012, grant Chercheuse d’avenir, Annette Vergunst)

Links

ZFIN database

IBCWG (International Burkholderia Cepacia Working group)